Background Current malaria diagnostic methods require blood collection, that may be associated with pain and the risk of transmitting blood-borne pathogens, and often create poor compliance when repeated sampling is needed. On the other hand, the collection of saliva is minimally invasive; but saliva has not been widely used for the diagnosis of malaria.

Aug 25, 2013 - Pages 229-237 Received 21 Jun 2012, Accepted 30 Apr 2013, Published online: 25 Aug 2013. Keywords: pollen analysis, Chaco region, Yunga region, native flora. In each independent experiment, the PTM voltage, gain and offset. Pollen associations from native plants, including Prosopis. People don't always say what's on their minds. One reason is that they are unwilling. For example, someone might report smoking a pack of cigarettes.

The aim of this study was to evaluate the diagnostic performance of saliva collected and stored at room temperature using the OMNIgene ®•ORAL kit for diagnosing Plasmodium falciparum malaria. Results Prevalence of malaria detected by TFM, nPCR-saliva and nPCR-blood was 22, 29, and 35%, respectively. Using TFM as the gold standard, the sensitivity of nPCR-saliva and nPCR-blood in detecting P. Falciparum was 95 and 100%, respectively; with corresponding specificities of 93 and 87%. When nPCR-blood was used as gold standard, the sensitivity of nPCR-saliva and microscopy was 82 and 68%, respectively; whereas, the specificity was 99 and 100%, respectively.

Nested PCR-saliva had a very good agreement with both TFM (kappa value 0.8) and blood PCR (kappa value 0.8). At parasitaemia > 10,000 parasites/µl of blood, the sensitivity of nPCR-saliva was 100%. Nested PCR-saliva detected 16 sub-microscopic malaria infections. One year after sample collection, P. Falciparum DNA was detected in 80% of saliva samples stored at room temperature. Plasmodium falciparum malaria remains one of the most important infectious diseases in sub-Saharan Africa (SSA).

Even with the significant reduction in malaria morbidity and mortality since the beginning of the third millennium, 214 million people (88% living in SSA) acquired malaria and 438,000 people (90% from SSA) died from malaria in 2015 [ ]. Transmission-blocking interventions, such as insecticide-treated nets, have been hailed as significant contributors to the decrease in the number of malaria cases and deaths. The proportion of children less than 5 years old in SSA who sleep under insecticide-treated nets increased from. Study design and population A cross-sectional study was conducted from October 2014 to April 2015 in selected health care facilities in three regions of Cameroon, i.e., the Far North, Centre, and Northwest regions. In the Far North region, the study was conducted in the Maroua regional hospital; the Catholic Health Center Nkolbisson was the study site in the Center region; while the PMI Nkwen Bamenda and the Mother of Mary Hospital Widikum were study sites in the Northwest region. Malaria transmission is perennial in both the Center and North West regions, but peak transmission occurs at different times of the year; whereas, malaria transmission is seasonal in the Far North region.